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. 2017 Oct 16;88:8–17. doi: 10.1016/j.curtheres.2017.10.003

Fig. 2.

Fig. 2

Western blotting (WB) analysis of the time-course sampling of the cell culture fluid of the COS-1 cells transfected with pCAG-CprME. The COS-1 cells were transfected with pCAG-CprME, lipofectamine (Lip) or Opti-MEM® medium (Thermo Scientific, Waltham, MA) as negative (Neg) control. The cell culture fluids were then collected at 24, 48, 72, and 96 hours post-transfection (hpt). An equal protein concertation of cell culture fluid samples (50 µg/20 µL) was then loaded on 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels and further analyzed by WB using a rabbit polyclonal anti-tick-borne encephalitis virus (anti TBEV) E protein serum.