Table.

Primers designed for amplification of CprME and prME of a Swedish strain of tick-borne encephalitis virus (Torö) and further cloning them into CAG and CMV plasmids. Annealing temperature used for polymerase chain reaction was 59˚C. Cleavage site for cloning are shown by small letter and underline.

Primer name	Sequence (5´→3´)	nt position	Length (nt)	GC (%)	Tm (˚C)
XCtbeF	AGAGctcgagATGGTCAAGAAGGCCATCC	133 (5´of C gene)	29	55	64.3
XAnchCtbeF	TATctcgagatgTCAGCGACGGACTGGATG	421 (5´of anchor C gene)	30	53	64.4
NEtbeR	AAATATAATgcggccgctaCGCCCCCACTCCAAG	2446 (3’ of E gene)	34	56	68.0

CprME, capsid prememberane envelope, GC, Guanine-Cytosine content, nt, Nucleotide (nt), pCAG, plasmid CMV early enhancer/chicken beta actin, pCMV, plasmid cytomegalovirus, prME, prememberane envelope, Tm, Melting temperature.