Figure 8:

2D ¹H-¹⁵N HSQC NMR titrations of PAR3G_EL (44–56) in the presence of ProT and PPACK-IIa. All NMR samples were in 25mM H₃PO₄, 150 mM NaCl, 0.2 inM EDTA and 10 % D₂O (pH 6.5). (A) For the PAR3G_EL binding studies with ProT, starting complexes included 50 μM PAR3G_EL (44–56, ¹⁵N-E48, ¹⁵N-L52) in 180 μM ProT. The serial dilutions resulted in ProT to PAR3G ratios that spanned from 4:1 to 0.1:1. (B) For the PPACK - IIa, starting complexes included 50 μM PAR3G_EL (44–56, ¹⁵N-E48,¹⁵N-L52) in 211 μM PPACK-Ha. The serial dilutions resulted in PPACK-Ha to PAR3G_EL ratios that spanned from 4:1 to 0.1:1. Representative data sets are shown. Colors for the 2D ¹H-¹⁵N HSQC crosspeaks span from blue (highest protein-peptide ratio) to red (free peptide).