Table 2.
Maximal growth (DCW), PF concentration (PFC), and specific PF productivity (YP/X) in the cultures during first medium screening in the Biolector® after culture for 48 h
| Medium | OTR = 30 mmol/L/h | OTR = 15 mmol/L/h | ||||
|---|---|---|---|---|---|---|
| Max. DCW (g/L) | Max. PFC (mg/L) | Max. YP/X (mg/g) | Max. DCW (g/L) | Max. PFC (mg/L) | Max. YP/X (mg/g) | |
| KB | 5.66 ± 0.12 | 32.8 ± 1.3 | 7.77 ± 0.55 | 5.11 ± 0.35 | 28.6 ± 0.9 | 6.82 ± 0.14 |
| KB-M1 | 5.17 ± 0.30 | 22.1 ± 0.9 | 5.03 ± 0.37 | 4.50 ± 0.15 | 18.7 ± 1.2 | 4.40 ± 0.37 |
| KB-M1 +Leu | 4.72 ± 0.43 | 57.9 ± 0.8 | 12.59 ± 0.41 | 4.51 ± 0.20 | 54.1 ± 0.5 | 12.38 ± 0.64 |
| KB-M1 +Glu | 5.25 ± 0.49 | 21.7 ± 1.3 | 6.46 ± 0.10 | 4.63 ± 0.26 | 20.0 ± 1.0 | 5.93 ± 0.24 |
| KB-M1 +AA mix | 3.97 ± 0.30 | 30.2 ± 0.8 | 8.18 ± 0.14 | 3.73 ± 0.37 | 28.8 ± 0.5 | 8.08 ± 0.27 |
| KB-M1 +Am | 4.25 ± 0.26 | 8.5 ± 0.9 | 2.02 ± 0.16 | 3.69 ± 0.49 | 7.6 ± 1.3 | 2.07 ± 0.35 |
| KB-M1 +Leu+Am | 4.76 ± 0.20 | 12.1 ± 1.0 | 2.68 ± 0.24 | 4.71 ± 0.07 | 10.2 ± 0.3 | 2.38 ± 0.14 |
| KB-M1 +Glu+Am | 4.95 ± 0.12 | 8.9 ± 1.3 | 2.02 ± 0.28 | 4.97 ± 0.23 | 8.1 ± 0.5 | 1.88 ± 0.05 |
| KB-M1 +Cit | 2.38 ± 0.39 | 2.4 ± 0.4 | 1.05 ± 0.22 | 2.47 ± 0.41 | 1.8 ± 1.0 | 0.72 ± 0.37 |
| KB-M1 +Suc | 3.32 ± 0.30 | 7.7 ± 0.2 | 2.72 ± 0.10 | 3.59 ± 0.18 | 3.2 ± 1.5 | 1.18 ± 0.52 |
All results are shown as the mean of three replicates ± SD. Culture media are summarized in Table 1 and in “Culture media” section
OTR oxygen transfer rate