Table 4.
Plackett–Burman screening design in the Biolector®
| Variable levels | Variable (units) | Max. DCW (g/L) | Max. PFC (mg/L) | Max. YP/X (mg/g) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| A | B | C | D | E | F | G | H | ||||
| Glycerol (g/L) | Tryptone (g/L) | Leu (g/L) | MgSO4 (g/L) | Fe2(SO4)3 (mg/L) | K2HPO4 (g/L) | Trace el. (mg/L) | OTR (mmol/L/h) | ||||
| Low (−): | 10 | 1 | 0.1 | 0.1 | 1 | 0.5 | 0.16 | 30 | |||
| Medium (0): | 55 | 8 | 2 | 1 | 50.5 | 2.75 | 1.68 | 45 | |||
| High (+): | 100 | 15 | 5 | 3 | 100 | 5 | 3.2 | 60 | |||
| Experimental run | |||||||||||
| 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 19.49 ± 0.23 | 134.2 ± 7.0 | 6.9 ± 0.4 |
| 2 | − | − | − | − | − | − | − | − | 4.81 ± 0.15 | 6.6 ± 0.6 | 1.4 ± 0.1 |
| 3 | + | − | − | − | + | + | + | − | 1.36 ± 0.06 | 4.3 ± 0.5 | 3.2 ± 0.3 |
| 4 | + | − | + | − | − | − | + | + | 4.61 ± 0.13 | 336.9 ± 7.0 | 73.2 ± 2.8 |
| 5 | − | + | + | + | − | + | + | − | 19.73 ± 0.42 | 55.0 ± 0.8 | 2.8 ± 0.1 |
| 6 | − | − | + | + | + | − | + | + | 11.17 ± 0.07 | 12.8 ± 0.7 | 1.1 ± 0.1 |
| 7 | + | − | + | + | − | + | − | − | 2.74 ± 0.04 | 129.4 ± 5.9 | 47.3 ± 2.3 |
| 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 19.49 ± 0.24 | 126.6 ± 7.2 | 6.5 ± 0.4 |
| 9 | − | + | + | − | + | − | − | − | 20.29 ± 0.18 | 132.7 ± 6.4 | 6.5 ± 0.3 |
| 10 | − | + | − | − | − | + | + | + | 16.19 ± 0.12 | 36.9 ± 0.7 | 2.3 ± 0.1 |
| 11 | + | + | − | + | + | − | + | − | 19.62 ± 0.19 | 412.4 ± 6.5 | 21.0 ± 0.3 |
| 12 | + | + | + | − | + | + | − | + | 23.14 ± 0.26 | 610.4 ± 5.9 | 26.4 ± 0.4 |
| 13 | − | − | − | + | + | + | − | + | 4.77 ± 0.07 | 1.0 ± 0.4 | 0.2 ± 0.1 |
| 14 | + | + | − | + | − | − | − | + | 18.66 ± 0.21 | 208.5 ± 6.5 | 11.2 ± 0.4 |
| 15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 19.43 ± 0.38 | 111.8 ± 6.5 | 5.8 ± 0.3 |
Results for each experimental run are shown as the mean of three replicates ± SD. Maximal growth (DCW), PF concentration (PFC), specific PF productivity (YP/X) after culture for 48 h. For further information on tested media, see “Culture media” section