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. 2018 Aug 4;16:59. doi: 10.1186/s12951-018-0386-6

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Cellular uptake of Ag-crosslinked α-DC-ZZ-BNC. a Isolated splenic DCs were incubated with each complex. The values were indicated percentages of fOVA⁺ cells in DCs. b The isolated DCs were treated with α-DC-ZZ-BNC-fOVA (green), and stained with wheat germ agglutinin (Red). Scale bars, 5 μm. c The DC2.4 cells were incubated with α-DC-ZZ-BNC-fOVA (green) and stained with LysoTracker Red (Red). Scale bars, 5 μm. Fluorescence intensity of fOVA in the DC2.4 cells was analyzed with an ImageJ software (N = 7, mean ± SEM)