Table 3.
klpΔ spore viability
| (A) Spore viability—zygotic asci
| ||||
|---|---|---|---|---|
| Strain | Spore viability
|
|||
| Wild type | klp5Δ | klp6Δ | klp5Δ, klp6Δ | |
| Wild type | 73% | |||
| klp5Δ | 74% | 1% | ||
| klp6Δ | 60% | 28% | 1% | |
| klp5Δ, klp6Δ | 25% | 1% | 5% | 2% |
| (B) Spore viability—azygotic asci
| ||||
|---|---|---|---|---|
| Strain | Spore viability
|
|||
| Wild type | klp5Δ | klp6Δ | klp5Δ, klp6Δ | |
| Wild type | 81% | |||
| klp5Δ | 60% | 2% | ||
| klp6Δ | 60% | 45% | 3% | |
| klp5Δ, klp6Δ | 25% | 9% | 2% | 1% |
(A) Spore viability from zygotic asci. Random spores from each cross were isolated, plated on YES medium, and colonies counted after 5 d. (B) Spore viability for azygotic asci. Stable diploids were isolated from each indicated cross, grown vegetatively to form colonies, and then induced to sporulate (see MATERIALS AND METHODS). Spore viability was then determined as described for zygotic crosses. Spores (1000) were plated for each cross.