Table 1.
Genotype | Parameters | No. of microtubulesa | |
---|---|---|---|
dhc1+ | Elongation rate (μm min−1) | 3.4 ± 1.4b | 76 |
Phase I | 3.5 ± 1.4c | 60 | |
Phase II | 3.2 ± 1.4c | 19 | |
Shortening rate (μm min−1) | 6.4 ± 3.4d | 61 | |
Phase I | 3.9 ± 6.2e | 48 | |
Phase II | 6.2 ± 3.1e | 15 | |
Maximum length (μm) | 8.2 ± 3.6f | 52 | |
Transition frequency (s−1) | |||
Elongation to shortening | 0.006g | 69 | |
Shortening to elongation | 0h | 44 | |
dhc1-d2 | Elongation rate (μm min−1) | 2.7 ± 0.8b | 22 |
Shortening rate (μm min−1) | 8.5 ± 2.2d | 27 | |
Maximum length (μm) | 5.3 ± 1.9f | 27 | |
Transition frequency (s−1) | |||
Elongation to shortening | 0.010i | 25 | |
Shortening to elongation | 0j | 26 |
Number of microtubules that were examined.
t test showed significant difference between values (P < 0.005).
t test showed no significant difference between values (P > 0.01).
t test showed significant difference between values (P < 0.001).
Forty-one transitions/6730 s of total elongation time.
Zero transitions/3423 s of total shortening time.
Seventeen transitions/1621 s of total elongation time.
Zero transitions/889 s of total shortening time.