Table 3.

Oligonucleotide primers used in this study

Primer name	Primer sequence (5′–3′)
A. Primers used in the PCR amplified disruption cassettes for gene disruption
D-ERG3_Fw	ATTTCTATCTTTCTTATCAATTCGTTTTTTCATTCACTTGTCAGCTGAAGCTTCGTACGC
D-ERG3_Rv	TCTTGAACGTGAAAGAAAGAAAAAAGATGAGACAAACAAGATAGGCCACTAGTGGATCTG
D-NCE103_Fw	TACAAATTTCAATTATTACACATCAGACAGCTGAAGCTTCGTACGC
D-NCE103_Rv	CCCCGTCTACTTTGTAAATGTCTTTCTATTTCAATGAATGGTAGGCCACTAGTGGATCTG
D-TRP1_Fw	GTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGACAGCTGAAGCTTCGTACGC
D-TRP1_Rv	CTATTTCTTAGCATTTTTGACGAAATTTGCTATTTTGTGCATAGGCCACTAGTGGATCTG
B. Primers used for the confirmation of successful gene disruption in yeast chromosome
C-ERG3_Fw1	CGAAACGACGCCTTTTGTTGCGATTGTCG
C-ERG3_Rv1	ATTTGTGTGCTTCTCTTGACGTTCGTTCG
C-ERG3_Fw2	TTCAACAAGTTTCAATAGCTCATCAGTCG
C-ERG3_Rv2	GAAATCTTGGGCATTTTAAAGCTTCCAGC
C-NCE103_Fw1	GTCACCATGACGCTTATCAAGCC
C-NCE103_Rv1	ATCGGGCGTTTACCGTATCGC
C-NCE103_Fw2	CTACACCTGGGGTCATGATTAGCC
C-NCE103_Rv2	GACATTTGCTGGATCACAGACCG
C. Primers used in the PCR amplification of the GAL1 promoter derivative fused with hCAII
pGAL1.4 Fw	TACAGCTAAGACTACAAAACGGATTAGAAGCCGCCG
pGAL1.3 Fw	TACAGCTAAGACTACAAACCGAGCGGGCGACAGCCC
pGAL1.2 Fw	TACAGCTAAGACTACAAACCGACGGAAGACTCTCCTC
pGAL1.1 Fw	TACAGCTAAGACTACAAAGCAGATGTGCCTCGCGCC
NotI_hCAII Rv	TTTTCCTTTTGCGGCCGCTTTTTTCCTTTTATTTATCATCATCATCTTTG