Skip to main content
. 2001 Dec;12(12):3973–3986. doi: 10.1091/mbc.12.12.3973

Figure 4.

Figure 4

Cell viability and microtubule structures after expression of GFP::TUB1-828 and GFP::TUB1. (A) Viability after transient expression of GAL1-driven TUB1 alleles. Raffinose cultures were treated with galactose then plated to glucose-containing plates. Viability is expressed in percentage of cells plated that resulted in colonies. Strain is DBY6597 containing pTS210 (GAL1p-vector), pRB2785 (GAL1p-TUB1), pRB2949 (GAL1p-TUB1-828), pRB2960 (GAL1p- GFP::TUB1), and pRB2963 (GAL1p- GFP::TUB1-828). (B) GFP-labeled microtubules after galactose-in-duction of GFP::TUB1 and GFP::TUB1-828 expression. Haploid spore clones from A (strains DBY9586-7) were grown in raffinose then treated with 2% galactose, 0.2% glucose for 3.5 h. Bar, 3 μm.