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. 2001 Dec;12(12):3973–3986. doi: 10.1091/mbc.12.12.3973

Figure 8.

Figure 8

Effect of nocodazole in cells expressing TUB1-828. Strains DBY9589 (GAL1p-TUB1) and DBY9590 (GAL1p-TUB1-828) were exposed to galactose for 2.5 h then placed onto a glucose medium containing 15 μg/ml nocodazole. (A) Visualization of GFP-labeled microtubules after placement of cells onto nocodazole. Time-lapse series are available in videos 8A_TUB1.mov (GAL1p-TUB1) and 8A_828.mov (GAL1p-TUB1-828). (B) Microtubule structures after 20-min exposure to nocodazole. Percentage of culture is indicated for each morphological class. At least 100 cells were counted for each strain. Arrow indicates microtubule unattached to spindle pole body. Bars, 3 μm.