Table 1.
Plasmids used in this study
| Name | Description | Source or reference |
|---|---|---|
| pTS210 | CEN-based vector with GAL1-10 promoter and ACT1 terminator | Marschall et al. (1996) |
| pTS408 | CEN-based vector with GFP under GAL1 promoter and ACT1 terminator | Carminati and Stearns (1997) |
| pTS988 | GFP(S65T)∷TUB1 integrative vector containing LEU2 | T. Stearns (Stanford University) |
| YIplac211 | Integrative vector containing URA3 | Gietz and Sugino (1988) |
| pRB300 | TUB3 in pBR322-derived vector | Schatz et al. (1986b) |
| pRB326 | TUB1 in CEN-based vector containing URA3 | Schatz et al. (1986b) |
| pRB327 | TUB1 in 2μ-based vector containing LEU2 | Schatz et al. (1986b) |
| pRB2065 | pUC119 vector containing TUB1 marked with flanking LEU2 integration | Richards et al. (2000) |
| pRB2311 | pRB2065 containing TUB1-820 | Richards et al. (2000) |
| pRB2332 | pRB2065 containing tub1-827 | Richards et al. (2000) |
| pRB2335 | pRB2065 containing TUB1-828 | Richards et al. (2000) |
| pRB2940 | pRB2065 containing TUB1-D252A | This study |
| pRB2942 | pRB2065 containing TUB1-E255A | This study |
| pRB2785 | TUB1 under GAL1 promoter (GAL1p-TUB1) | This study |
| pRB2945 | TUB3 integrative vector containing URA3 | This study |
| pRB2947 | TUB3-E255A integrative vector containing URA3 | This study |
| pRB2949 | TUB1-828 under GAL1 promoter (GAL1p-TUB1-828) | This study |
| pRB2951 | TUB1-D252A under GAL1 promoter (GAL1p-TUB1-D252A) | This study |
| pRB2953 | TUB1-E255A under GAL1 promoter (GAL1p-TUB1-E255A) | This study |
| pRB2956 | TUB1-820 under GAL1 promoter (GAL1p-TUB1-820) | This study |
| pRB2958 | tub1-827 under GAL1 promoter (GAL1p-tub1-827) | This study |
| pRB2960 | GFP∷TUB1 under GAL1 promoter (GAL1p-GFP∷TUB1) | This study |
| pRB2963 | GFP∷TUB1-828 under GAL1 promoter (GAL1p-GFP∷TUB1-828) | This study |
| pRB2804 | GFP(S65T)∷TUB1 under ACT1 promoter in CEN-based plasmid containing LEU2 | This laboratory |