Table 3.
Yeast strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| FY2 | MAT a ura3-52 | Winston et al. (1995) |
| FY23 | MAT a ura3-52 leu2-Δ1 trp1-Δ63 | Winston et al. (1995) |
| DBY2637 | MAT a his2-Δ200 leu2-3,112 lys2-801 ura3-52 tub1Δ∷HIS3 tub3Δ∷TRP1 [pRB327] | This laboratory |
| DBY6596 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB1–LEU2/tub1Δ∷HIS3 [pRB326] | Richards et al. (2000) |
| DBY6597 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 [pRB326] | This laboratory |
| DBY6600 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB1–LEU2/TUB1–LYS2 [pRB326] | Richards et al. (2000) |
| DBY6627 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 tub1-827–LEU2/TUB1–LYS2 [pRB326] | Richards et al. (2000) |
| DBY6628 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB1-828–LEU2/TUB1–LYS2 [pRB326] | Richards et al. (2000) |
| DBY8247 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB1-820–LEU2/TUB1–LYS2 [pRB326] | Richards et al. (2000) |
| DBY8915 | MAT a ade2-(ΔEcoRV - Stu1) lys2-ΔEcoRV leu2-Δ1 ura3-52 | This laboratory |
| DBY9557 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB1-D252A–LEU2/TUB1–LYS2 [pRB326] | This study |
| DBY9559 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB1-E255A–LEU2/TUB1–LYS2 [pRB326] | This study |
| DBY9561 | MAT a his2-Δ200 leu2-3,112 lys2-801 ura3-52 tub1Δ∷HIS3 tub3Δ∷TRP1 [pRB2785] | This study |
| DBY9562 | MAT a his2-Δ200 leu2-3,112 lys2-801 ura3-52 tub1Δ∷HIS3 tub3Δ∷TRP1 [pRB326] | This study |
| DBY9563 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB3-E255A/TUB3 [pRB327] | This study |
| DBY9565 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB3-E255A/TUB3 [pRB2785] | This study |
| DBY9566 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB3/TUB3 [pRB2785] | This study |
| DBY9574 | MAT a ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 TUB3 [pRB2785, pRB2804] | This study |
| DBY9576 | MAT a ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 TUB3-E255A [pRB2785, pRB2804] | This study |
| DBY9578 | MAT a/α ade2-101/ade2-101 his3-Δ200/his3-Δ200 lys2-801/lys2-801 leu2-Δ1/leu2-Δ1 ura3-52/ura3-52 TUB3-E255A/TUB3 [pRB326] | This study |
| DBY9579 | MAT a ade2-(ΔEcoRV - Stu1) leu2-Δ1 ura3-52 | This study |
| DBY9580 | MAT a ade2-(ΔEcoRV - Stu1) leu2-Δ1 ura3-52 GFP(S65T)∷TUB1–LEU2 | This study |
| DBY9583 | MAT a ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 [pTS210] | This study |
| DBY9584 | MAT a ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 [pRB2785] | This study |
| DBY9585 | MAT a ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 [pRB2949] | This study |
| DBY9586 | MAT a ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 [pRB2960] | This study |
| DBY9587 | MAT a ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 [pRB2963] | This study |
| DBY9589 | MAT a ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 [pRB2785, pRB2804] | This study |
| DBY9590 | MAT a ade2-101 his3-Δ200 lys2-801 leu2-Δ1 ura3-52 [pRB2949, pRB2804] | This study |