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Efficacy of extracts from Eucommia ulmoides Oliv. on the balance of RANKL and OPG. Representative immunohistochemical staining obtained from ankle joints of the several groups, (a) on RANKL detection and (b) on OPG detection. (c) AOD value of RANKL or OPG was analyzed using Image-Pro Plus software. (d) The relative RANKL/OPG ratio was calculated based on AOD values of RANKL and OPG. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 compared with CIA group.
