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. Author manuscript; available in PMC: 2019 Jan 9.
Published in final edited form as: Nat Methods. 2018 Jul 9;15(8):617–622. doi: 10.1038/s41592-018-0044-9

Figure 3. Characterization of the mitochondrial proteome.

Figure 3

(a) New mitochondrial proteins not containing an N’ MTS that could only be visualized when N’ tagged with NOP1pr-GFP (Left) or were already visible under the NATIVEpr-GFP (Right). (b) The NATIVEpr-(NATIVE)MTS-GFP library (Not including Su9-MTS) uncovered new mitochondrial proteins containing an N’ MTS. (c) Several proteins can still be seen on mitochondria even after swapping their NOP1pr-Su9MTS-GFP cassette to a TEF2pr-mCherry without an MTS, suggesting alternate targeting information is found in these proteins. (d) MTS and TMD prediction analysis for proteins showing mitochondrial localization with the NOP1pr-GFP, NATIVEpr-GFP, and/or C’ GFP tags. Cx(9)C - cysteine-rich domain 26, β-barrel domain 27. All scale bars are 5µm. Imaging of strains was performed a single time. Images represent entire field.