Table 3.
Mutational analysis of adenine binding site: uncoupling of ATPase activity and microtubule gliding in vitro
| Condition | kcat s−1 | Km (MT) μM | MANT-ATP/ATP %a | Motility rate nm · s−1 |
|---|---|---|---|---|
| GST-K333 | 29.8 | 0.24 | ||
| GST-K333RFAP | 10.2 | 0.66 | ||
| GST-K333REAP | 2.8 | 0.72 | ||
| K401B | 17.3 | 0.2 | 100 | 670 |
| K401BRFAP | 11.7 | 1.6 | 81 | 1.3 |
| K401BREAP | 2.6 | 1.8 | 50 | N.D. |
| NOD485B | 22 | 0.5 | 0.8 | N.D. |
N.D., none detected.
a
Phosphate release was measured in the presence of either 0.2 mM MANT-ATP or 0.2 mM ATP. Shown is the ratio of phosphate release in the presence of MANT-ATP versus ATP.