Fig. 6.

Representative images of GFAP+ astrocytes in the hippocampus of adult Long-Evans hooded male rats exposed to low-F⁻ chow/RO-H₂O or low-F⁻ chow/20 ppm F⁻ drinking water beginning on gestational day 6. (a) Suprapyramidal blade of the dentate gyrus. (b) CA1 pyramidal cell layer. Cells displayed normal process-bearing morphology with no evidence of hypertrophy. 3,3-diaminobenzidine staining (brown). Hematoxylin counterstain (blue) showed no disruption of the normal morphology of the hippocampal regions and no evidence of neuronal death. (n = 6). Scale bar = 100 μm