Figure 5.

Recognition of Glutamate Receptor Family Members by GluA4-Targeted Vector Particles

To determine the selectivity of the GluA4-targeted vector particles, CHO-K1 cells expressing GluA1-4 or GluR6 as well as the parental cell line were incubated with the indicated AAV (top panel) and LV (bottom panel) particles, respectively. In all experiments, the cells were analyzed for GFP expression 72 hr post-transduction by flow cytometry. Untransduced cells, AAV particles displaying the EpCAM-specific DARPin Ec1 (top panel), or LV particles displaying the GluA2- and GluA4-specific scFv Fab7 (bottom panel) were used as control. Each transduction experiment was performed at least three times with individually produced vector particles, showing mean values, SDs, and p values. ns, not significant; p values by one-way ANOVA comparing each condition with each other per vector sample (Tukey’s multiple comparisons test). See also Figure S5.