Figure 2.

Proteomic cargo of circulating exosomes was determined by LC-MS/MS. (A) Number of proteins shared by osteosarcoma (OSA), dogs with traumatic fracture (FX), and healthy controls (N) illustrated with a Venn diagram. (B) A supervised partial least squares discriminate analysis exhibits a distinct separation of the three groups based on their exosomal proteomic signature. (C) The 10 most significant proteins for discrimination between osteosarcoma (OSA) and fracture (FX) or normal (N) are listed along with relative protein expression (red = high, blue = low) (Table 1). OSA appears to have a distinct profile when compared with the other two groups, demonstrating primarily upregulated protein expressions.