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. 2018 Jul 30;9:1705. doi: 10.3389/fimmu.2018.01705

Figure 6.

Figure 6

4M2e-tFliC microneedle patch (MNP) immune sera play a protective role in heterologous virus infection. BALB/c mice (N = 5) were intramuscular (IM) immunized twice with the divalent inactivated vaccine (DIV). One group of immunized mice was boosted with 4M2e-tFliC MNPs for the third immunization (DIV + MNPs). Three weeks after the MNP vaccination, the other two groups of DIV-immunized mice were intraperitoneally (IP) injected with naïve serum (DIV + Naïve serum) or 4M2e-tFliC immune serum (DIV + 4M2e-tFliC serum), respectively. Naïve mice received 4M2e-tFliC immune serum as a control (Naïve + 4M2e-tFliC serum). (A,B) Body weight change after 2 × LD50 of A/Vietnam/1203/2004 (H5N1, rVet) and A/Shanghai/02/2013 (H7N9, rSH) reassortant virus challenges. (C,D) Lung viral titers at day 5 post H5N1 and H7N9 challenges. Data represent mean ± SEM. The statistical significance was analyzed by Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, n.sp > 0.05.