Table 1.
Summary of DIY bioreactor and microplates.
Name | In-line detection | Volume | Organism | Note | Reference |
---|---|---|---|---|---|
Turbidostat | OD, fluorescence | 15 ml | Yeast | 3D printed holder and pump | Takahashi et al., 2014 |
Morbidostat | OD | 12 ml | E. coli | Antibiotic drug resistance | Toprak et al., 2012, 2013; Liu et al., 2016 |
Microreactor | OD, PH, and oxygen | 1 ml | E. coli | Point of care | Lee et al., 2011; Perez-Pinera et al., 2016 |
Optogenetic bioreactor | OD fluorescence | 12 ml | E. coli | Optogenetics | Wang and Yang, 2017 |
Light tube array (LTA) | None (fluorescence with offline flow cytometry) | 1 ml | E. coli | Optogenetics for bacteria and mammalian cell entrapment of cyanobacteria | Olson et al., 2014; Gerhardt et al., 2016 |
Cell growth quantifier (CGQ) | OD | 50 ml | Yeast | Back scattering and high speed data acquisition | Bruder et al., 2016 |
Microplate with robotic assay | OD, fluorescence | ∼100 μl | E. coli | Gene expression profiling | Zaslaver et al., 2006, 2009; Huber et al., 2009 |
Optical Microplate | None (fluorescence with offline flow cytometry) | 100 ml | E. coli Dunaliella tertiolecta | Photosynthesis optogenetics | Chen et al., 2012; Davidson et al., 2013; Lee et al., 2013 |
PhotobioBox | OD | 200 ml | Microalgae | Light intensity and temperature gradient between the wells | Heo et al., 2015 |
Several design features of each solution are listed for comparisons, e.g., working volume, organism the cultivation is designed for an in-line detection capabilities. Under the “Note” column we have listed the main intended application or any special features of the solution.