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. 2018 Jul 30;11:263. doi: 10.3389/fnmol.2018.00263

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Copyright © 2018 Yang, Seo, Oh, Ryu, Kim, Lee, Ryu and Choe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phosphorylation of GluA1-S831 after repeated cocaine administration in the Nucleus accumbens (NAc). (A) Timeline for western immunoblotting analysis. (B) Brain sections showing statistics for area punched out for western immunoblotting analysis (blue circles). (C) GluA1-S831 phosphorylation was significantly increased at 30 min after repeated cocaine administration and sustained up to 60 min as compared with the saline control group. This increase was returned to the basal level at the 120 min time point (Interaction, F_(5,30) = 16.54, df = 5, p < 0.05; Time, F_(5,30) = 16.54, df = 5, n = 4 rats per group; p < 0.05; two-way repeated measures analysis of variance (RM ANOVA) followed by Bonferroni’s multiple comparisons test. *p < 0.05 vs. repeated saline group; ^#p < 0.05 vs. repeated cocaine group at the 30 min time point. Western immunoblotting was performed repeatedly four times.