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. 2018 Jul 30;9:1586. doi: 10.3389/fimmu.2018.01586

Figure 6.

Figure 6

Figure 6

Differential mRNA expression profile of A549 cells challenged with pre-incubated (A) pH1N1, (B) H3N2 with rfhSP-D, and (C) expression levels of type I interferon (IFN) subtypes in both untreated and treated samples. The expression levels of cytokines and chemokine were measured using qRT-PCR and the data were normalized via 18S rRNA expression as a control. The relative expression (RQ) was calculated by using cells only time point as the calibrator. The RQ value was calculated using the formula: RQ = 2−ΔΔCt. Assays were conducted in triplicates and error bars represents ± SEM. Significance was determined using the unpaired one-way ANOVA test (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001) (n = 3).