Figure 1.

KIF1A Binds to TANC2, Liprin-α, and CaM

(A) bioGFP-KIF1A(383-881, 657-1105, 657-1698, 950-1250, 1105-1698) used to perform AP-MS experiments.

(B) KIF1A interactors identified by MS. p values and spectral counts are graphically represented by colors and spheres, respectively. See also Figures S1A and S1B and Tables S1 and S2.

(C) TANC1 and TANC2 spectral counts detected by MS.

(D) TANC (TANC1 Ab, PanTANC Ab, and TANC2 Ab) immunoprecipitation-MS experiments. p values and spectral counts of KIF1A are graphically represented by colors and spheres, respectively.

(E) Western blots (WBs) of HA-TANC proteins and liprin-α1 in AP experiments of bioGFP-KIF1A(657-1105, 657-1698) from co-transfected HEK293 cells.

(F) WB of HA-liprin-α2 in AP of bioGFP-KIF1A(657-1105, 657-1698).

(G and H) WB of HA-KIF1A in AP of bioGFP-TANC2 (G) or bioGFP-liprin-α2 (H).

(I) WB of HA-KIF1A in AP of full-length GFP-TANC2 and GFP-TANC2 fragments (1-832, 833-1227, 1228-1500, and 1501-1900).

(J) WB of HA-KIF1A in AP of full-length GFP-liprin-α2 and GFP-liprin-α2 fragments (1-735 and 796-1257).

(K) Schematic representation of KIF1A in association with the PSD scaffolding proteins TANC2 and liprin-α2 and with the calcium sensor CaM.

(L) WB of HA-CaM in AP of bioGFP-KIF1A fragments (657-1105, 1105-1698, and 657-1698).