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. 2018 Jul 17;24(3):557–568.e5. doi: 10.1016/j.celrep.2018.06.080
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Mouse anti-GFP (clones 7.1 and 13.1) Roche 11814460001
Mouse monoclonal anti-GAPDH (clone 6C5) Millipore Sigma MAB374
Rabbit polyclonal anti-Gαolf Corvol et al., 2001 N/A

Chemicals, Peptides, and Recombinant Proteins

Dulbecco’s modified Eagle’s medium Thermo Fisher Scientific 11965-092
Fetal bovine serum Millipore Sigma 12303C
Sodium pyruvate Thermo Fisher Scientific 11360-070
MEM non-essential amino acids Thermo Fisher Scientific 11140-050
Penicillin-streptomycin Thermo Fisher Scientific 15140-122
Puromycin Thermo Fisher Scientific A1113803
Neurobasal-A medium Thermo Fisher Scientific 10888-022
GlutaMAX supplement Thermo Fisher Scientific 35050-061
B-27 Supplement Thermo Fisher Scientific 17504044
Hanks’ balanced salt solution Thermo Fisher Scientific 14175-095
Papain Worthington Biochemical LS003126
DNase I Thermo Fisher Scientific 18047019
Poly-D-lysine hydrobromide Millipore Sigma P6407
Matrigel Corning 356230
Dulbecco’s phosphate-buffered saline Millipore Sigma D8537
Hanks’ balanced salt solution Millipore Sigma 55037C
CO2-independent medium Thermo Fisher Scientific 18045-088
cOmplete, EDTA-free protease inhibitor cocktail Millipore Sigma 11873580001
Dynabeads Protein G Thermo Fisher Scientific 10004D
Dopamine hydrochloride Millipore Sigma H8502

Critical Commercial Assays

GloSensor cAMP Reagent Promega E1290
Nano-Glo Luciferase Assay Substrate (furimazine) Promega N1120
NucleoSpin RNA Plus Kit Takara Bio 740984.10
Titanium One-Step RT-PCR Kit Takara Bio 639503

Experimental Models: Cell Lines

Human: HEK293T/17 ATCC CRL11268
Human: GNAS knockout HEK293T/17 This study N/A

Experimental Models: Organisms/Strains

Mouse: CAMPER Muntean et al., 2018 N/A
Mouse: Drd1-Cre JAX Tg(Drd1-cre)EY262Gsat
Mouse: C57BL/6 CRL C57BL/6NCrl

Oligonucleotides

Primer: Gαolf forward: GATCGAGAAGCAGTTGCAGAAAGAG This study N/A
Primer: Gαolf reverse: CTTTGTCCACTTGGAATCGTGTCTC This study N/A
Primer: GAPDH forward: GTCTTCACCACCATGGAGAAGG This study N/A
Primer: GAPDH reverse: GAAGGCCATGCCAGTGAGCTTC This study N/A

Recombinant DNA

Plasmid: GNAS CRISPR guide RNA pLentiCRISPR v2 This paper N/A
Plasmid: Dopamine D1 receptor cDNA Resource Center DRD0100000
Plasmid: Gαolf cDNA Resource Center GNA0L00000
Plasmid: Gαolf P102_V104del This paper N/A
Plasmid: Gαolf F133L Dos Santos et al., 2016 N/A
Plasmid: Gαolf V137M This paper N/A
Plasmid: Gαolf V146M This paper N/A
Plasmid: Gαolf E155K This paper N/A
Plasmid: Gαolf V172I This paper N/A
Plasmid: Gαolf R188C This paper N/A
Plasmid: Gαolf G213S This paper N/A
Plasmid: Gαolf Q214L cDNA Resource Center GNA0L000C0
Plasmid: Gαolf V228F This paper N/A
Plasmid: Gαolf V234I This paper N/A
Plasmid: Gαolf S239N This paper N/A
Plasmid: Gαolf A311T This paper N/A
Plasmid: Gαolf R329W Masuho et al., 2016 N/A
Plasmid: Gαolf A353T This paper N/A
Plasmid: Gαolf V354A This paper N/A
Plasmid: Venus 156-239-Gβ2 This paper N/A
Plasmid: Gβ2 cDNA Resource Center GNB0200000
Plasmid: Venus 1-155-Gγ7 Lohmann et al., 2017 N/A
Plasmid: Gγ7 cDNA Resource Center GNG0700000
Plasmid: Flag-tagged Ric-8B Von Dannecker et al., 2006 N/A
Plasmid: PTX-S1 Raveh et al., 2010 N/A
Plasmid: masGRK3ct-Nluc Masuho et al., 2015b N/A
Plasmid: CalfluxVTN Himmelreich et al., 2017 N/A
Plasmid: pGloSensor-22F cAMP Promega E2301
Plasmid: mTrpM3α2-C-GFP Ghosh et al., 2016 N/A
Plasmid: masGRK3ct Kammermeier et al., 2000 N/A
Plasmid: pmCherry-N1 Vector Takara Bio 632523

Software and Algorithms

R version 3.4.2 R Core Team https://www.r-project.org/
ImageJ National Institute of Health SCR_003070
GraphPad Prism 6 Graphpad Software SCR_002798
SigmaPlot 12.5 SYSTAT Software SCR_003210
PyMol Schrödinger SCR_000305
Clampfit 10.3 Molecular Devices SCR_011323
T-Coffee Notredame et al., 2000 SCR_011818
BoxShade SCR_007165

Other

GPCRdb (Pándy-Szekeres et al., 2018 http://gpcrdb.org/
gnomAD Lek et al., 2016 http://gnomad.broadinstitute.org/
REVEL Ioannidis et al., 2016 https://sites.google.com/site/revelgenomics/downloads
ANNOVAR Wang et al., 2010 http://annovar.openbioinformatics.org/en/latest/