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EMSAs for CBF3CCΔN
(A) Fluorescently labeled CDEIII DNA (1.6 μM) and a titration of CBF3CCΔN (lanes 2–4) or Cep3ΔN (lanes 5–7) or 6.4 μM of full-length Cep3 (lane 10). Lanes 8 and 9 show competition of CBF3CCΔN binding with 80 μM unlabeled probe.
(B) Fluorescently labeled CDEIII DNA (1.6 μM) with a titration of CBF3CCΔN (lanes 2–6) and CBF3CCΔN with Ctf13 mutations R307A-R308A-R330A.
See also Figure S6.
