Figure 2.

WIP Binding to Actin Influences PI3K Signaling and BCR-Induced Actin Reorganization

(A) Immunoblot of splenic WT or WIPΔABD B cells stimulated with immobilized anti-κ-chain antibody on beads and probed with antibodies as indicated. Quantifications of intensity of proteins normalized by densitometry to tubulin and to the signal in unstimulated WT cells at t = 0 on the right. This experiment and the experiment in Figure S3F were performed simultaneously. The loading control measurements were part of both experiments. Data (mean ± SEM) are representative of at least two independent experiments.

(B) Flow cytometric analysis of intracellular phalloidin of WT and WIPΔABD B cells. Quantification of the geometric mean fluorescence intensity (gMFI) is shown on the right (mean ± SEM).

(C) Representative SIM images of phalloidin staining indicating the intracellular amount of F-actin in fixed WT or WIPΔABD B cells settled on coverslips coated with anti-κ-chain antibody for 10 min. Scale bar, 4 μm. Quantification (right graph) shows F-actin foci per cell (mean ± SEM). Data are representative of three independent experiments.

See also Figure S2.