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. 2018 Jul 30;9:1723. doi: 10.3389/fmicb.2018.01723

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Copyright © 2018 Zhang, Zong, Wang, Zhu, Hu, Li, Zhang, Chen, Liu and Tan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induction of cytokine mRNA and protein expression in SC19-infected J774A.1 macrophages with or without fisetin treatment. J774A.1 macrophages were treated with 32 μg/ml fisetin in the absence (negative control) or presence of SC19 for 5 h. (A) The mRNA levels of IL-1β and TNF-α were determined by qRT-PCR. (B) The cytokine protein levels in the supernatants were determined by ELISAs. The bars represented the SEMs, on the basis of three independent experiments. Statistically significant differences are indicated. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001.