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. 2018 Jul 30;9:1764. doi: 10.3389/fimmu.2018.01764

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Copyright © 2018 Borkner, Misiak, Wilk and Mills.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Azithromycin (AZM) treatment during immunization attenuates the protective efficacy of a whole cell pertussis (wP) vaccine. Mice were treated with AZM or vehicle during immunization with wP vaccine 6 and 2 weeks before aerosol challenge with Bordetella pertussis. Results are mean ± SEM, CFUs in lung homogenates of four mice per group at each timepoint. Statistical significances were determined via two-way ANOVA followed by Tukey’s multiple comparisons test, only VAC compared to VAC + AZM is indicated, significances for comparison between NON VAC and VAC/VAC + AZM was highly significant (***, not indicated), ***p < 0.001.