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Image analysis to access cell proliferation and protein expression. (A) The proliferating cells were labeled with EdU in both control and BKM120 treated groups. (B) The expression of phosphorylated Akt were stained by immunofluorescence in both control and BKM120 treated groups. (C) Quantitative analysis of positive cells per area unit regarding of the proliferating cells and the expression of phosphorylated Akt in both control and BKM120 treated groups.
