Figure 4.

Production of some proinflammatory cytokines at the acute stage of Neospora caninum infection. Wild-type (WT), Nlrp3^−/−, Asc^−/−, and Caspase-1/11^−/− mice were infected with 1 × 10⁷ of N. caninum tachyzoites (intraperitoneal route) for 7 days. IL-1β (A), IL-18 (B), IFN-γ (C), IL-12 (D), IL-6 (E), and TNF-α (F) were measured by ELISA in serum of infected mice. Data are shown as the mean ± SEM from two independent experiments (A,B) or from three independent experiments (C–F) (*P < 0.05, **P < 0.01, ***P < 0.001 vs. WT group).