Figure 6.

Recruitment of monocytes/macrophages and neutrophils at the acute stage of Neospora caninum infection. Wild-type (WT), Nlrp3^−/−, Asc^−/−, and Caspase-1/11^−/− mice were infected with 1 × 10⁷ of N. caninum tachyzoites (intraperitoneal route) for 7 days, and peritoneal exudate cells were collected for flow cytometry analysis. (A). Total numbers of peritoneal exudate cells in each infected mouse were counted. (B–D) The influx of monocytes/macrophages and neutrophils (CD11b⁺Ly6C⁺ cells) was evaluated by flow cytometry. Monocytes/macrophages (Ly6C⁺Ly6G⁻ cells) and neutrophils (Ly6C⁺Ly6G⁺ cells) in peritoneal exudate cells were analyzed by flow cytometry (E), and their numbers are shown (F,G). Data are shown as the mean ± SEM from three independent experiments (A) or are representative of two independent experiments (B–G) (*P < 0.05, **P < 0.01, ***P < 0.001 vs. WT group).