Figure 3.

Macrophage polarization in epididymal adipose tissue of GHSR knockout mice. The mRNAs were extracted from the epididymal adipose tissue harvested from the GHSR knockout mice and wild type mice fed with different diet. Real-time PCR was performed to evaluate the expression of macrophage specific markers. (a) M₁ markers MCP-1, TNF-α, iNOS and (b) M₂ markers Arg-1, Mgl-1, Mrc1 were detected. Data are means±SEM n=5. ∗ denotes P<0.05 compared with WT mice fed with NCD; ^# denotes P<0.05 compared with WT mice fed with HFD.