Fig. 2.

EPS protects V. cholerae against T6SS attacks from heterologous organisms. (A) Competition between V. cholerae V52 expressing the chimeric VgrG3-NucSe1 nuclease effector from S. enterica subsp. arizonae and T6SS⁻ V52vasK, with or without the vpsA deletion. (B) Competition between A. baylyi ADP1 and T6SS⁻ V52vasK, with and without the vpsA deletion. (C) Competition between P. aeruginosa PAO1 and T6SS⁺ V52 and T6SS⁻ V52vasK, each with or without the vpsA deletion. (D) Competition between T6SS⁺ V52 and T6SS⁻ V52vasK, each with or without the vpsA deletion, against E. coli MG1655. Data represent the mean ± SD of three biological replicates. Student’s t test was performed to compare each condition (ns, P > 0.05; *P ≤ 0.05; **P ≤ 0.01).