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. 2018 Jul 18;115(31):7997–8002. doi: 10.1073/pnas.1808469115

Fig. 4.

Fig. 4.

Up- or down-regulation of EPS genes result in corresponding increase or decrease in resistance to T6SS-dependent killing. (A) Expression of EPS production genes vpsA and vpsL in flaA and motX mutants of V. cholerae V52 were measured by qRT-PCR. (B) P. aeruginosa PAO1 was competed against T6SS+ V52 and T6SS− V52 vasK with no additional mutations, deletion of vpsA, deletion of flaA, or deletion of motX. (C) Expression of vpsA and vpsL genes required for EPS production in flaA and motX mutants of V. cholerae 2740-80 were measured by qRT-PCR. (D) P. aeruginosa PAO1 was competed against T6SS+ 2740-80 and T6SS− 2740-80 vipA with no additional mutations, deletion of vpsA, deletion of flaA, deletion of flaA vpsA, or deletion of motX. Data represent the mean ± SD of three biological replicates. Student’s t test was performed to compare each condition (ns, P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001, ****P ≤ 0.0001).