Figure 5.

Distribution of telomere-like sequences in yeast. (A) Distribution of telomere-like DNA, sequences that match the C_1–3A/TG_1–3 consensus sequence for yeast telomeric DNA, within the distal ∼220 kb of the test chromosome VII. Only tracts of nine bases or longer that are in the correct orientation to support telomere addition are shown. The most distal 9- and 11-base pair tracts are from URA3 and were only on the test copy of chromosome VII. When URA3 is in its normal position near the centromere of chromosome V, these 9- and 11-base pair tracts are not in the same orientation as telomeric DNA. There was one 9-base pair tract of telomere-like DNA on the endogenous copy of chromosome VII that was eliminated during construction of the test chromosome. (B) Exact sequence and position of 9-base pair or longer tracts of telomere-like DNA in the same (left) or different (right) orientation as telomeric DNA are shown. The coordinates for each tract are based on the sequence of intact chromosome VII, which is ∼15 kb longer than the test chromosome. (C) Presence of tracts of telomere-like DNA of 15 bps or greater in the yeast genome. Chromosomes I (230 kb), III (317 kb), IX (440 kb), X (745 kb), and XI (670 kb) had no telomere-like tracts >14 bps. Only tracts in the same orientation as telomeric DNA are shown. The subset of C_1–3A/TG_1–3 tracts that are (CA)_n DNA are marked by arrows. All tracts >35 base pairs are adjacent to Y′ subtelomeric repeat sequences, with the exception of the (TG)₃₁ tract located around position 210 kb on chromosome VI. ♦, 15–19 base pairs; □, 20–24 base pairs; ▵, 25–29 base pairs; ●, 35–39 base pairs; *, >50 base pairs; ↓, CA tracts.