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. 2018 Jul 18;115(31):E7351–E7360. doi: 10.1073/pnas.1800039115

Fig. 7.

Fig. 7.

Loss of CIP2A impacts proliferation and anchorage-dependent growth. (A) Knockdown of CIP2A with three different siRNAs reduces the expression of Sox2 in MycN-amplified neuroblastoma cell lines. (B) SK-N-AS cells with wild-type MycN status express undetectable levels of MycN or Sox2 but substantial levels of cMyc that are unaffected by the knockdown of CIP2A. (C) The PLA shows cobinding of MycN and CIP2A. (Scale bars: 50 μm.) (D) Knockdown of CIP2A reduces relative viability in neuroblastoma cell lines [SK-N-BE(2), P = 0.00040 and 0.01201; SK-N-AS, P = 0.019, t test]. (E) Loss of CIP2A also decreased anchorage-dependent growth in SK-N-BE(2) cells (P = 0.0049 and 0.0068). *P < 0.05, **P < 0.01.