Table 1.
Mutagenesis analysis of ClpP
| Mutant # | Amino acids | Activity | M21 inhibition |
| 1 | P116A | + | C |
| 2 | N117A | +/− | |
| 3 | A118G | ++ | R |
| 4 | E119A | — | |
| 5 | V120A | ++ | C |
| 6 | M121A | / | |
| 7 | I122A | +/− | |
| 8 | H123A | — | |
| 9 | E135A | — | |
| 10 | I136A | — | |
| 11 | E137A | +++ | C |
| 12 | I138A | +++++ | S |
| 13 | A139G | ++++ | C |
| 14 | A140G | — | |
| 15 | N141A | — | |
| 16 | H142A | — | |
| 17 | I143A | — | |
| 18 | L144A | — | |
| 19 | K145A | +++ | C |
| 20 | T146A | +++ | R |
| 21 | R147A | — | |
| 22 | E148A | ++++++ | C |
| 23 | D170A | — | |
| 24 | R171A | — | |
| 25 | D172A | — | |
| 26 | N173A | +/− | S |
| 27 | F174A | — | |
| 28 | L175A | / | |
| 29 | T176A | +++ | S |
| 30 | A177G | + | C |
| 31 | Wild-type | +++ | C |
C, similar as wild-type ClpP control; S, sensitive than wild-type ClpP control; R, resistance than wild-type ClpP control.
+With enzyme activity. The more pluses, the higher activity.
−No enzyme activity.
/Did not get mutant.