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. 2018 Jul 16;115(31):8019–8024. doi: 10.1073/pnas.1805538115

Fig. 4.

Fig. 4.

JUN is O-GlcNAcylated at Ser84, Thr131, Ser132, Ser138. (A) Western blot analysis of JUN O-GlcNAcylation. Flag-tagged versions of JUN wild-type (WT) and Ala substitution mutants (S84A, T131A, S132A, S138A, and 4O; all four residues converted to Ala) were expressed in HEK 293T cells expressing exogenous OGT and treated with Thiamet-G. Immunoblot analysis of immunoprecipitated FLAG-JUN for O-GlcNAcylation (anti-O-GlcNAc RL2) and total JUN (anti-FLAG). (B) Schematic representation of JUN O-GlcNAcylation. Phosphorylation sites (blue), four O-GlcNAcylation sites (red), and basic leucine zipper domain (bZIP) were depicted.