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. 2018 Jun 25;115(31):E7331–E7340. doi: 10.1073/pnas.1805757115

Fig. 8.

Fig. 8.

Kv2 interaction with VAPs in rat hippocampal neurons. (A) Coexpression of Kv2.1-loopBAD and VAPA-GFP. Surface Kv2.1-loopBAD was visualized with CF640-conjugated streptavidin. (B) Coexpression of GFP-Kv2.2 and VAPB-mRuby2. (C–E) Colocalization of Kv2.1-loopBAD, GFP-Kv2.2, and the CD4-Kv2.1:573–589 chimera with VAPA-GFP, VAPB-mRuby2, and VAPA-GFP, respectively, within the AIS. The AIS was confirmed with anti-neurofascin antibody staining as illustrated in SI Appendix, Fig. S2. (Scale bars: 5 μm.) (F) Summary of the percentage of neurons concentrating VAPs at induced ER/PM junctions. Error bars indicate SEM. P values comparing the interaction of the first three CD4-Kv2.1 chimeras with either VAPA or VAPB to WT Kv2.1 were not significant, with Kruskal–Wallis ANOVA values of P = 0.37 and P = 0.1, respectively.