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. 2018 Jul 30;9:1100. doi: 10.3389/fpls.2018.01100

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Copyright © 2018 Silva, Guirgis and Thornburg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of nectary-expressed carotenoids from different species of Nicotiana sp. (A) Silica gel thin-layer chromatogram (TLC) of carotenoids isolated from stage 12 nectaries of N. glauca; N. benthamiana; N. clevelandii; N. plumbaginifolia; N. rustica; N. bonariensis and N. alata; N. langsdorffii; and N. sylvestris. The origin, solvent front, and migration of various pigments are indicated. Solvent used was 9:1 acetone: hexane solvent. (B) Absorbance (450 nm) of β-carotene in nectary extracts at stage 12. Averages ± standard deviation (N = 3). Species evaluated: 1, N. glauca; 2, N. benthamiana; 3, N. clevelandii; 4, N. plumbaginifolia; 5, N. rustica; 6, N. bonariensis; 7, N. alata; 8, N. langsdorffii; and 9, N. sylvestris. Different letters mean statistical differences between groups.