FIGURE 2.

Mild ER stress increased p-IRE1α expression but had no effect on CHOP expression in primary cultured astrocytes. (A) Astrocytes were stained with p-IRE1α antibody. p-IRE1α-immunopositivity in primary astrocytes was observed using confocal scanning. (B) Astrocytes were stained with CHOP antibody. CHOP-immunopositivity in primary astrocytes was observed using confocal scanning. Blue staining represents DAPI. Scale bar = 25 μm. (C) Quantitative data of the mean intensity of p-IRE1α fluorescence in primary astrocytes. (D) Quantitative data of the mean intensity of CHOP fluorescence in primary astrocytes. Each value was then expressed relative to that of the control group, which was set to 100. All experiments were repeated three times. ^∗P < 0.05, ^∗∗P < 0.01 vs. control group. The data are presented as the mean ± SEM.