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. 2018 Mar 28;10(7):891–901. doi: 10.2217/epi-2018-0016

Figure 1. . Locations of the CpG sites detected by HM450k and Sequenom EpiTYPER.

Figure 1. 

In the validation study with the Sequenom EpiTYPER assay, four DNA amplicons were involved; amplicon 1 covered three CpG sites (CpG1 to CpG3) which were located in the TSS1500 region of the GRHL3 gene. Amplicon 2 and amplicon 3 covered the CpG island region of GRHL3 predicted using UCSC (chr1:24648202–24648985). These two amplicons included 32 analytical CpG sites (CpG4 to CpG35). Amplicon 4 covered six CpG sites (CpG36 to CpG41) within the gene body. Nucleotide positions accord to the NCBI build 37/hg19. The differentially methylated CpG units identified by 450 K Array (cg27298252, cg24860886, cg18196063, cg20325200 and cg16734875) are also indicated.