Figure 3. . Phenotype analysis of GRHL3-overexpression and grhl3-MO in zebrafish embryos.
(A) Phenotype analysis of zebrafish embryos treated with GRHL3 mRNA: based on the phenotype severity, we divided the phenotypes into four grades: grade 1: no difference comparing with wild-type, no shortening of the body axis; grade 2: mild deformity, curling body axis with the length reduced about 1/4 relative to the wild-type; grade 3: moderate deformity, severe curvature and body axis shortened nearly 1/2; grade 4: severe deformity, no body axis. (B) Phenotype analysis of zebrafish embryos after microinjection with GRHL3 mRNA ranging from 100 to 300 pg. The abnormal rates of overexpressed groups were significantly higher than control group. With an increase of the mRNA, the malformation rate showed a rising tendency. (C) Phenotype analysis of MO experiment. Distributions of these four grades of the different dose groups at 24 hpf and 48 hpf were analyzed separately.
*p < 0.05 by χ2 test.
MO: Morpholino.