Table 1. . Methylation of GRHL3 gene using the Human Methylation 450 Bead Chip assay.
| Target ID | Chr | Mapinfo | Case | Control | Diff | ad-p | Region |
|---|---|---|---|---|---|---|---|
| cg24860886 | 1 | 24645397 | 0.205 | 0.546 | -0.341 | 0.005† | TSS1500 |
| cg27298252 | 1 | 24645380 | 0.876 | 0.930 | -0.054 | 0.048† | TSS1500 |
| cg06376426 | 1 | 24648203 | 0.675 | 0.720 | -0.045 | 0.285 | TSS1500 |
| cg21273275 | 1 | 24648328 | 0.367 | 0.283 | 0.084 | 0.310 | TSS1500 |
| cg09322349 | 1 | 24645184 | 0.532 | 0.601 | -0.069 | 0.438 | TSS1500 |
| cg22176324 | 1 | 24648851 | 0.072 | 0.069 | 0.003 | 0.722 | TSS1500 |
| cg05036846 | 1 | 24648984 | 0.073 | 0.072 | 0.001 | 0.911 | TSS1500 |
| cg14616251 | 1 | 24648696 | 0.076 | 0.076 | 0.000 | 0.995 | TSS1500 |
| cg13987674 | 1 | 24649494 | 0.854 | 0.913 | -0.059 | 0.106 | TSS200 |
| cg09662754 | 1 | 24645805 | 0.081 | 0.099 | -0.018 | 0.188 | TSS200 |
| cg12757705 | 1 | 24645802 | 0.051 | 0.057 | -0.006 | 0.464 | TSS200 |
| cg11709405 | 1 | 24649398 | 0.394 | 0.448 | -0.055 | 0.489 | TSS200 |
| cg15050111 | 1 | 24645850 | 0.075 | 0.073 | 0.002 | 0.817 | TSS200 |
| cg20242797 | 1 | 24645853 | 0.114 | 0.118 | -0.005 | 0.852 | TSS200 |
| cg18196063 | 1 | 24652248 | 0.811 | 0.891 | -0.080 | 0.012† | 5′UTR |
| cg26365938 | 1 | 24645953 | 0.077 | 0.089 | -0.012 | 0.074 | 5′UTR |
| cg21289280 | 1 | 24645885 | 0.049 | 0.058 | -0.010 | 0.166 | 5′UTR |
| cg12405265 | 1 | 24655961 | 0.843 | 0.833 | 0.010 | 0.678 | 5′UTR |
| cg04552500 | 1 | 24645917 | 0.099 | 0.095 | 0.004 | 0.711 | 5′UTR |
| cg23820945 | 1 | 24646022 | 0.145 | 0.153 | -0.008 | 0.906 | 5′UTR |
| cg14167629 | 1 | 24646046 | 0.117 | 0.105 | 0.011 | 0.659 | First exon |
| cg20325200 | 1 | 24667622 | 0.590 | 0.812 | -0.222 | 0.000† | Body |
| cg16734875 | 1 | 24678010 | 0.883 | 0.943 | -0.060 | 0.011† | Body |
| cg19697558 | 1 | 24646085 | 0.070 | 0.068 | 0.002 | 0.916 | Body |
| cg16155382 | 1 | 24646191 | 0.129 | 0.101 | 0.028 | 0.373 | Body |
| cg01119512 | 1 | 24646205 | 0.230 | 0.201 | 0.029 | 0.408 | Body |
| cg02984188 | 1 | 24646392 | 0.364 | 0.482 | -0.118 | 0.240 | Body |
| cg11155431 | 1 | 24664451 | 0.886 | 0.890 | -0.004 | 0.830 | Body |
Target ID is identified according to HM450k. The nucleotide position is based on NCBI build 37/hg19. Region is defined relative to the nearest open reading frame: within 1500 (TSS1500) or 200 bp (TSS200) of a transcription start site, in the 5′ UTR, the first exon of a transcript (exon) and in the body of gene (body). Differentially methylated CpG sites were identified by two criterions: the false discovery rate <0.05, which was analyzed by independent t-tests with multiple comparison tests; the absolute β-value difference >0.0516.
†The CpG site was aberrant methylated in case group.
ad-p: Adjusted p-value; Chr: Chromosome; diff: Difference; Mapinfo: Nucleotide position; UTR: Untranslated region.