Figure 5. . Survival curves and concentration responses for gallic acid, hexyl gallate, octyl gallate and dodecyl gallate from the zebrafish model.

The graph represents combined data expressed as mean and standard error mean from three independent experiments. Survival curves were plotted using the Kaplan–Meier method, and survival curves were compared by log-rank test. The group of untreated embryos is represented by the black line and the different concentrations of compounds are represented by the colored lines. Gallic acid was not toxic to the embryos at all concentrations tested, and gallates were toxic in a concentration-dependent manner. The compounds significantly reduced the survival rate of embryos at concentrations ≥5 μg/ml compared with control (p < 0.05) for hexyl and octyl gallates, and ≥15 μg/ml compared with control (p < 0.05) for dodecyl gallate.

DOD: Dodecyl gallate; GAL: Gallic acid; HEX: Hexyl gallate; OCT: Octyl gallate.