Table 1.
Amino acids sequence and charge distribution of OEP7wt and mutants
| OEP7 | mEsvakpattkEgsakqaaivvgvlalgwfaiqvafiplfnkvrgggsDkkDDDvnaftpDt |
| − + +− + TM ++ −++−−− − | |
| OEP7-Δ4 | mEsvakpattkEgsakqaaivvgvlalgwfaiqvafiplfnkvrgggsDkkDDDvlrt |
| − + +− + TM ++ −++−−− + | |
| OEP7-Δ12 | mEsvakpattkEgsakqaaivvgvlalgwfaiqvafiplfnkvrgggsDk |
| − + +− + TM ++ −+ | |
| OEP7-LM1 | mEsvakpattkqgkakqaaivvgvlalgwfaiqvafiplfnkvrgggsDkkDDDvnaftpDt |
| − + + ++ TM ++ −++−−− − | |
| OEP7-LM2 | mEsvakpattkEgsakqaaivvgvlalgwfaiqvafiplfnkvEgggsDkkDDDvnaftpDt |
| − + +− + TM +− −++−−− − | |
| OEP7-LM3 | mEsvakpattkqgkakqaaivvgvlalgwfaiqvafiplfnkvEgggsDkkDDDvnaftpDt |
| − + + ++ TM +− −++−−− − |
| N-terminus ↓ | 4aa ↓ ← | 4aa → ↓ | C-terminus ↓ | ||
|---|---|---|---|---|---|
| OEP7 | +1 | +1 | charge of the flanking regions | +2 | −1 |
| OEP7-Δ4 | +1 | +1 | +2 | +1 | |
| OEP7-Δ12 | +1 | +1 | +2 | +2 | |
| OEP7-LM1 | +3 | +2 | +2 | −1 | |
| OEP7-LM2 | +1 | +1 | ±0 | −3 | |
| OEP7-LM3 | +3 | +2 | ±0 | −3 |
Capital letter, acidic amino acid; italic letter, basic amino acid; underlined letter, predicted TM domain; bold letter (OEP7), charged amino acid; bold letter (OEP7 mutant), point mutation; aa, amino acid.