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. 2018 Jul 13;201(4):1315–1326. doi: 10.4049/jimmunol.1800323

FIGURE 1.

FIGURE 1.

Avid binding of human mAbs to CSP constructs. (A) Schematic representation of CSP and the peptides NANP6 and PF16 corresponding to the central repeat and C-terminal regions of CSP, respectively. (B and D) Specific binding of C-terminal region targeting human mAb AB236 IgG1 to C-terminal region peptide PF16 (B) and CSP-FL (D), respectively, is shown. (C and E) The NANP repeat–targeting mAb AB334 IgG1 binding to NANP6 peptide (C) and rCSP-FL (E), respectively, is displayed. Blue lines in the panels indicate the association and dissociation of Abs at various indicated concentrations, which were globally fitted (red lines) to obtain association rate, dissociation rate, and Kd values shown in (B)–(E). In (B)–(E), cartoons display the BLI assay configuration of CSP Ag constructs loaded onto biosensors that were dipped into Abs placed in wells of a 384-well microplate. Ag immobilization levels were 1 nm for PF16 (B), 0.01 nm for NANP6 (C), and 0.1 nm for CSP-FL (D and E). Abs were diluted into kinetics buffer for measuring binding to NANP6 and CSP-FL Ags (C–E). PBS buffer was used to record AB236 binding to PF16.