Table I. Repeatability of k_a, k_d, and K_d measurements of CSP Ag (at high density) binding of anti-CSP mAbs in PBS buffer and 1:50-diluted NHS measured in triplicate.

Ag	Ab	Diluent	Mean ka (×105 M−1 s−1)	Mean kd (×10−4 s−1)	Mean Kd (nM)
PF16	AB236	PBS	1.82 ± 0.08 (%CV = 4.4)	1.98 ± 0.29 (%CV = 14.6)	1.09 ± 0.19 (%CV = 17.4)
PF16	AB236	NHS	2.20 ± 0.02 (%CV = 0.9)	3.90 ± 0.19 (%CV = 4.9)	1.77 ± 0.08 (%CV = 4.5)
NANP6	AB334	PBS	0.817 ± 0.002 (%CV = 0.2)	<0.01a	<0.01a
NANP6	AB334	NHS	1.02 ± 0.01 (%CV = 1.0)	<0.01a	<0.01a
CSP-FL	AB236	PBS	4.62 ± 0.10 (%CV = 2.2)	<0.01a	<0.01a
CSP-FL	AB236	NHS	6.33 ± 0.91 (%CV = 14.4)	<0.01a	<0.01a
CSP-FL	AB334	PBS	0.57 ± 0.03 (%CV = 5.3)	<0.01a	<0.01a
CSP-FL	AB334	NHS	0.37 ± 0.04 (%CV = 10.8)	<0.01a	<0.01a

^aSD and %CV were ND because of slower than resolvable dissociation rate (k_d < 0.01 × 10⁻⁴ s⁻¹).