Table II. Intermediate precision of ka, kd, and Kd measurements of CSP Ag binding (at high density) of CSP-specific mAbs in PBS and 1:50-diluted NHS.
| Ag–Ab/Diluent | Parameter | Operator 1 Mean ± SD n = 3 | Operator 2 Mean ± SD n = 3 | Equipment 1 Mean ± SD n = 3 | Equipment 2 Mean ± SD n = 3 | Day 1 Mean ± SD n = 3 | Day 2 Mean ± SD n = 3 |
|---|---|---|---|---|---|---|---|
| PF16- | ka (×105 M−1 s−1) | 1.53 ± 0.08 | 1.36 ± 0.05 | 1.82 ± 0.08 | 1.48 ± 0.03 | 1.48 ± 0.03 | 1.53 ± 0.08 |
| AB236/ | kd (×10−4 s−1) | 1.98 ± 0.34 | 2.03 ± 0.52 | 1.98 ± 0.29 | 1.65 ± 0.56 | 1.65 ± 0.56 | 1.98 ± 0.34 |
| PBS | Kd (nM) | 1.30 ± 0.28 | 1.50 ± 0.41 | 1.09 ± 0.19 | 1.12 ± 0.39 | 1.12 ± 0.39 | 1.30 ± 0.28 |
| PF16- | ka (×105 M−1 s−1) | 2.22 ± 0.07 | 2.12 ± 0.03 | 2.20 ± 0.02 | 2.33 ± 0.11 | 2.33 ± 0.10 | 2.22 ± 0.07 |
| AB236/ | kd (×10−4 s−1) | 4.41 ± 0.60 | 4.54 ± 0.51 | 3.90 ± 0.19 | 3.62 ± 0.47 | 3.62 ± 0.47 | 4.41 ± 0.60 |
| NHS | Kd (nM) | 1.99 ± 0.28 | 2.15 ± 0.26 | 1.77 ± 0.01 | 1.56 ± 0.23 | 1.56 ± 0.23 | 1.99 ± 0.28 |
| NANP6- | ka (×105 M−1 s−1) | 0.83 ± 0.04 | 0.817 ± 0.002 | 0.817 ± 0.002 | 0.814 ± 0.006 | 0.817 ± 0.002 | 0.777 ± 0.004 |
| AB334/ | kd (×10−4 s−1) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| PBS | Kd (nM) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| NANP6- | ka (×105 M−1 s−1) | 0.90 ± 0.02 | 1.02 ± 0.01 | 1.02 ± 0.01 | 1.05 ± 0.01 | 1.02 ± 0.01 | 0.948 ± 0.006 |
| AB334/ | kd (×10−4 s−1) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| NHS | Kd (nM) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| CSP-FL- | ka (×105 M−1 s−1) | 5.22 ± 0.51 | 5.17 ± 0.28 | 4.69 ± 0.12 | 4.63 ± 0.10 | 4.69 ± 0.12 | 5.22 ± 0.52 |
| AB236/ | kd (×10−4 s−1) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| PBS | Kd (nM) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| CSP-FL- | ka (×105 M−1 s−1) | 6.41 ± 0.37 | 8.01 ± 0.37 | 6.33 ± 0.91 | 5.91 ± 1.00 | 5.91 ± 1.00 | 6.41 ± 0.38 |
| AB236/ | kd (×10−4 s−1) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| NHS | Kd (nM) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| CSP-FL- | ka (×105 M−1 s−1) | 0.55 ± 0.04 | 0.589 ± 0.005 | 0.56 ± 0.02 | 0.57 ± 0.03 | 0.56 ± 0.02 | 0.55 ± 0.04 |
| AB334/ | kd (×10−4 s−1) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| PBS | Kd (nM) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| CSP-FL- | ka (×105 M−1 s−1) | 0.366 ± 0.008 | 0.391 ± 0.007 | 0.366 ± 0.004 | 0.369 ± 0.002 | 0.369 ± 0.002 | 0.366 ± 0.008 |
| AB334/ | kd (×10−4 s−1) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
| NHS | Kd (nM) | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |